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Recombinant Human BRAK/CXCL14
Synonyms: Breast and Kidney-expressed chemokine, CXCL14, bolekine, NJAC
Description: Breast and Kidney-expressed chemokine (BRAK) is a CXC chemokine expressed in
normal tissue in the absence of inflammatory stimuli, and infrequently expressed in cancer cell lines.
BRAK is known to be a highly selective monocyte chemoattractant. However, main function and
receptor selectivity is unknown at this time. BRAK contains the four highly conserved cysteine residues
present in CXC chemokines. The sequence of the mature protein consists of 87 amino acid residues,
and is approximately 30% homologous to the sequences of MIP-2 alpha and beta. Recombinant human
BRAK is a 9.4 kDa protein containing 77 amino acid residues.
Catalog #: 300-50 Lot #: 060191
Source: E.coli
Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses
Endotoxin level: Endotoxin level is less than 0.1 ng per mg (1EU/mg).
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of
0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and
stored at 4oC for 1 week or -20oC for future use.
Stability: The lyophilized protein is stable for a few weeks at room temperature, but best
stored at -20oC. Reconstituted BRAK should be stored in working aliquots at -
20oC.
Biological Activity: Determined by its ability to chemoattract activated monocytes using a
concentration range of 1.0-10.0 ng/ml.
For a list of references using this product please visit our website at www.peprotech.com
Usage: For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Country of Origin: USA
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