Product Information Recombinant Human GROa/MGSA/CXCL1 Synonyms: Growth Regulated Protein/Melanoma Growth Stimulatory Activity, GROa, MGSAa, CXCL1, NAP-3, GRO1, KC (murine) CINC (rat) Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. GRO/MGSA also stimulates mitogenesis in certain human melanoma cells. Recombinant human GRO/MGSA is a 7.8 kDa protein consisting of 73 amino acids including the ‘ELR’ motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. Source: E.coli Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses Endotoxin level: Endotoxin level is less than 0.1 ng per mg (1EU/mg). Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20oC. Reconstituted GRO/MGSA should be stored in working aliquots at -20oC. Biological Activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-50.0 ng/ml. For a list of references using this product please visit our website at www.peprotech.com Usage: For Research Use Only. Not for use in diagnostic or therapeutic procedures. Country of Origin: USA